The milestone has been achieved whilst still delivering consistently high levels of customer service that have been recognised by industry awards.
The Docklands Light Railway (DLR) carried one hundred million passengers this past financial year - breaking all its previous records and exceeding the numbers being carried on some mainline services.
The DLR's Head of Planning, Robert Niven, said: 'This significant milestone caps a magnificent year for the DLR which saw us carry 7.2m people during the 2012 Olympic Games - double what we would normally carry during that time.
'And in just 25 years, the DLR has grown from running 11 trains on just two routes to becoming a key part of the world's most prestigious sporting event while also servicing a rapidly growing local community.
'We have managed our expansion and increased our capacity to these unforeseen levels all while maintaining some of the highest reliability scores in the UK. We are now well placed to meet exciting future challenges as the east and south east of London host new developments and become the job creation hotspots of the next decades.'
The DLR was opened by the Queen in August 1987, with 11 trains serving 15 stations and in its first year of operation it carried 6.7m people.
Today the railway - which is entirely step-free - has 45 stations, 46 km of track and 149 carriages.
The network was one of Britain's first light rail systems, and it has one of the safest and most advanced automatic train control systems in the world.
Since opening, it has been extended to Bank, Beckton, Lewisham and Woolwich Arsenal.
During the 16 days of the London 2012 Olympic Games, 7.2 million passengers were carried on the DLR - up 100 per cent on normal levels; on 3 August 2012, Day 7 of the Games, the DLR carried over 500,000 daily passengers - its busiest day ever.
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